COMPARATIVE MAPPING OF HUMAN CHROMOSOME 17 GENES IN PIGS AND SHREWS
Larkin D.M.*, Kuznetsov S.B., Ivanova E.V., Astakhova N.M., Serov O.L.,
Zhdanova N.S.
Institute of Cytology and Genetics, Russian Academy of Sciences, Novosibirsk, Russia
Comparative mapping approach for establishing of chromosomal relationships of genes across the species, enables to reveal evolutionary conserved chromosomal segments and provides valuable information about genomic evolution.
We present results of mapping of human chromosome 17 (HSA17) genes in pigs (Artiodactyla) and common shrew (Insectivora). Zoo-FISH analyses showed HSA17 homology with pig chromosome 12 (SSC12) and h-arm of shrew chromosome hn. We tested a set of human, mice, and bovine PCR primers used for mapping of bovine homolog of HSA17 [1] in shrews and pigs. 5 of 19 tested primer pairs amplified single product of expected size in pigs and 3 of 20 – in shrews. Besides, we have developed consensus PCR primers for ENO3 basing on human and mice sequences, for THRA1 using human and porcine sequences. As a result of pig-mink and pig-hamster somatic hybrids analyses, genes for ENO3, THRA1, CRYB1, LIS1, MYL4 were assigned to porcine chromosome 12 preliminary to q arm, gene for PRKCA to 12p14-cen, gene P4HB to 12pter-p15. Genes for NF1, MYL4, and THRA1 we assigned to shrew chromosome hn using a set of shrew-mice somatic hybrids. PCR product of shrew THRA1 was partially sequenced and showed 95% homology with sheep and human THRA1 under NCBI BLAST program.
Results obtained confirm the homology between HSA17, SSC12, and common shrew chromosome hn. By the other way, SSC12 contains intrachromosomal rearrangements resulting in changing of gene order in comparison with HSA17. The possibility of using of Zoo-PCR analysis for mapping of syntenic gene groups has being shown.
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